Cardiac-mimetic cell culture platform and a method for direct cardiac reprogramming

• Main Authors: KIM BYUNG SOO, CHAR KOOKHEON, SONG SEUK YOUNG, YOO JIN
• Format: Patent
• Language: English; Korean
• Published: 30.07.2021
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a cell differentiation method and a cell differentiation apparatus, capable of increasing efficiency of direct differentiation of stem cells into cardiomyocytes by simulating a cardiac microenvironment through electrical stimulation and cell co-culture. The cell differentiation method according to the present invention, by simultaneously applying two factors, electrical stimulation and co-culture, in vivo microenvironment of the cardiomyocytes can be simulated to further increase differentiation efficiency. Therefore, the present invention can be utilized in various studies such as treatment of cardiovascular diseases including myocardial infarction. 본 발명은 전기자극 및 세포 공배양을 통하여 심장 미세환경을 모사함으로써, 줄기세포를 심근세포로 분화시키는 직분화 효율을 높일 수 있는 세포 분화 방법 및 세포 분화 장치에 관한 것이다. 본 발명에 따른 세포 분화 방법은 전기적 자극과 공배양 두 가지 요소를 동시에 적용시킴으로서 심근세포의 생체 내 미세환경을 모사하여 분화 효율을 더욱 높일 수 있다. 따라서 본 발명은 심근경색을 포함한 심혈관 질환의 치료 등 다양한 연구에 활용될 수 있다.