Separation and purification method of maltodextrin using reverse phase chromatography

• Main Authors: KWAK, JI YUN, YOU, MIN JEE
• Format: Patent
• Language: English; Korean
• Published: 01.02.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; POLYSACCHARIDES; SEPARATION; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; TRANSPORTING; VINEGAR; WINE

The present invention relates to a separation and purification method for maltodextrin using reversed-phase chromatography and, specifically, to a separation and purification method for maltodextrin, capable of separating the maltodextrin in high purity of minimum 83% or greater by each molecule, wherein the separation and purification method for maltodextrin is a method for separating and purifying the maltodextrin manufactured by an enzymatic method, by a polymerization size of glucose using reversed-phase chromatography. According to the present invention, the separation and purification method for maltodextrin enables the injection of a sample of high concentration in comparison with existing size exclusion chromatography. The separation and purification method for maltodextrin can effectively separate the maltodextrin by having excellent separation performance even if the maltodextrin is separated for short time. Also, the separation and purification method for maltodextrin can easily remove a reaction by-product. Therefore, the separation and purification method for maltodextrin can be usefully used for industrial production of the maltodextrin of the high purity by producing a single kind of the maltodextrin with the high purity in a large quantity. 본 발명은 역상 크로마토그래피를 이용하는 말토덱스트린의 분리정제 방법에 관한 것으로, 상세하게는 효소적 방법에 의해 제조된 말토덱스트린을 역상 크로마토그래피를 이용하여 말토덱스트린을 포도당의 중합 크기별로 분리 및 정제하는 방법이며, 각각의 분자별로 최소 83% 이상의 고순도로 분리할 수 있는 말토덱스트린의 분리정제 방법에 관한 것이다. 본 발명의 제조방법은 종래에 사용되던 크기 배제 크로마토그래피(size exclusion chromatography)에 비하여 고농도의 샘플주입이 가능하며, 분리능이 우수하여 짧은 시간 동안 분리하여도 효율적으로 분리할 수 있으며, 반응 부산물의 제거도 쉽게 이루어질 수 있다. 따라서 고순도의 단일 종류의 말토덱스트린을 대량 생산할 수 있으므로 고순도 말토덱스트린의 산업적 생산에 유용하게 활용될 것이다.