Acidified seawater responsive genes in Aurelia aurita and the prediction method for physiological or metabological changing of Scyphozoa

• Main Authors: LEE, AE KYUNG, SHIN, KYOUNG SOON, LEE,YOUNG, YUM, SEUNG SHIC
• Format: Patent
• Language: English; Korean
• Published: 21.03.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to an Aurelia aurita gene responding to acidified seawater exposure, and to a method for diagnosing a change in an aquatic ecosystem environment by using the same. In particular, a polyp of Aurelia aurita is cultured, and is exposed to acidified seawater of pH 7.5 for 6, 24, and 48 hours. RNA is extracted therefrom, and cDNA is synthesized. Then, hybridization is performed by marking the same by Cy3 and Cy5, and a microarray analysis is performed by utilizing an Aurelia aurita gene Hazchem fish array. As a result, it has been confirmed that the expression of 33 genes in an acidified seawater (pH 7.5) 6-hour exposure group, the expression of 36 genes in a 24-hour exposure group, and the expression of 96 genes in a 48-hour exposure group are changed by two or more times. Also, it has been confirmed that the expression amounts of total 165 genes excluding overlapping genes are changed by exposure to acidified seawater. Accordingly, the genes can be usefully used as a biomarker for confirming the exposure of acidified seawater, and can be usefully utilized in predicting the hypertrophied of jellyfish according to a change in the marine environment. 본 발명은 산성화된 해수 노출에 대응하는 보름달 물해파리 유전자 및 이를 이용한 수생태계 환경변화를 진단하는 방법에 관한 것으로, 구체적으로 보름달물해파리의 폴립을 배양하여 pH7.5의 산성화된 해수에 6, 24 및 48시간 동안 노출시킨 후, 이로부터 RNA를 분리하고, cDNA를 합성한 뒤, Cy3 및 Cy5로 표지하여 혼성화 하고, 보름달물해파리 유전자 해즈켐 피쉬 배열(HazChem Fish Array)를 활용한, 마이크로어래이 분석을 실시한 결과, 산성화 해수(pH 7.5) 6시간 노출군에서는 33종의 유전자; 24시간 노출군에서는 36종의 유전자; 48시간 노출군에서는 96종의 유전자가 2배이상 발현이 변화되는 것을 확인하였고, 중복되는 유전자들을 제외한 총 165종의 유전자들이 산성화된 해수의 노출에 의해 발현량이 변화되는 것을 확인함으로써, 상기 유전자들은 산성화 해수의 노출을 확인할 수 있는 바이오마커로 유용하게 활용될 수 있으며, 해양환경 변화에 따른 해파리의 대발생 예측에 유용하게 활용될 수 있다.