Nonylphenol responsive genes in Oryzias javanicus and the method for diagnosing aquatic environment pollution using the same

• Main Authors: WOO, SEON OCK, JO, YE JIN, YUM, SEUNG SHIC
• Format: Patent
• Language: English; Korean
• Published: 06.01.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to an Oryzias javanicus gene responsive to nonylphenol (NP) exposure, and to a method for diagnosing aquatic environmental contamination by using the same. In particular, Oryzias javanicus is cultured, and is exposed to nonylphenol at a concentration of 20 g/L for 12 hours, 24 hours, 48 hours, and 72 hours, and RNA is separated therefrom. Then, cDNA is synthesized and marked by Cy3 and Cy5 before performing hybridization. An oligo-microarray is produced and analyzed by utilizing an EnviHaz-Fish array. As a result, it has been confirmed that an expression amount of 123 genes is changed by twice or more compared to a control group by the exposure of nonylphenol, using heterogeneous microarray experimental analysis. Accordingly, the genes can be useful as biomarkers for confirming nonylphenol exposure. 본 발명은 노닐페놀(Nonylphenol, NP) 노출에 대응하는 바다송사리() 유전자 및 이를 이용하여 수생태계 환경오염을 진단하는 방법에 관한 것으로, 구체적으로 바다송사리()를 배양하여 노닐페놀을 20 ㎍/L농도로 12, 24, 48, 및 72시간 동안 노출시킨 후, 이로부터 RNA를 분리하고, cDNA를 합성한 뒤, Cy3 및 Cy5로 표지하여 혼성화하였고, oligo-마이크로어래이를 제작하여 앤비헤즈 피쉬 배열(EnviHaz-Fish Array)를 활용하여 이를 분석한 결과, 이종간의 마이크로어래이 실험분석을 이용하여, 노닐페놀의 노출에 의해 123종의 유전자 발현량이 대조군 대비 2배이상 변화하는 것을 확인함으로써, 이들 유전자들은 노닐페놀 노출여부를 확인할 수 있는 바이오마커로 유용하게 사용될 수 있다.