METHOD FOR PRODUCING COAT PROTEIN OF HANTAN VIRUS SP. AND TRANSFORMED MICROORGANISM PRODUCING THE SAME

• Main Authors: PARK, WAN JE, NOH, GAP SU, KIM, SU OK, LEE, SEONG HUI, HONG, SEON PYO, KIM, HYEON SU, YOO, WANG DON, SHIN, YEONG CHEOL, HA, SEOK HUN
• Format: Patent
• Language: English; Korean
• Published: 14.01.2002
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PURPOSE: A method for producing a coat protein of hantan virus sp. and a transformed microorganism producing the same are provided. The coat protein of hantan virus sp. can be useful in the production of a hantan virus vaccine. CONSTITUTION: A method for producing a coat protein of hantan virus sp. comprises the steps of: cloning the coat protein of hantan virus sp. G1 gene, G2 gene or G1G2 gene fused with other protein genes into an appropriate vector; transforming a host microorganism with the recombinant plasmid constructed; culturing the transformant under appropriate conditions; purifying the expressed fusion protein; and treating the purified fusion protein with an appropriate factor to remove proteins fused with the coat protein, wherein protein fused with the coat protein is maltose binding protein; the vector is pMalc2 or pMalp2; the host microorganism is E. coli; and the recombinant plasmid is pMalc2-G1, pMalc2-G2, pMalc2-G12, pMalp2-G1, pMalp2-G2 or pMalp2-G12.