A GREEN FLOURESENCE SILWORM USING BY THE RECOMBINANT BACULOVIRUS CONTAINING THE GREEN PROTEIN GENE AND ITS METHOD

PURPOSE: Provided are the fluorescent silkworms which light green fluorescence by illumination of ultraviolet rays. And a producing method thereof using a recombinant baculovirus containing the green fluorescent protein gene is also provided. Wherein, the green fluorescent protein gene can be transf...

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Bibliographic Details
Main Authors YUN, EUN YOUNG, KIM, KEUN YOUNG, JIN, BYUNG RAE, KANG, SEOK KWON, YOON, HYUNG JOO, KANG, SEOK WOO, KIM, HO RAK
Format Patent
LanguageEnglish
Published 01.11.2000
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE: Provided are the fluorescent silkworms which light green fluorescence by illumination of ultraviolet rays. And a producing method thereof using a recombinant baculovirus containing the green fluorescent protein gene is also provided. Wherein, the green fluorescent protein gene can be transferred into the next generation of silkworms. CONSTITUTION: The fluorescent silkworms are produced by the steps of: producing the recombinant autographa californica nuclear polyhedrosis virus(AcNPV) containing the green fluorescent protein gene which is positioned under regulation of polyhedrosis protein gene promoter by inserting the 720 bp of green fluorescent protein gene into baculovirus; and infecting the recombinant AcNPV into silkworms and feeding the silkworms at 25 deg. C, in which the silkworms show the green fluorescence in breast 4 days after injection and show the green fluorescence in total body 7 days after injection.
Bibliography:Application Number: KR19980000444