NEW PROTEASE

• Main Authors: DAMBMANN, CLAUS, OUTTRUP, HELLE, AASLYNG, DORRIT A, CHRISTIANSEN, MARGRETHE
• Format: Patent
• Language: English; Korean
• Published: 15.02.2000
• Edition: 7
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; BEER; BIOCHEMISTRY; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENT COMPOSITIONS; DETERGENTS; ENZYMOLOGY; FATTY ACIDS THEREFROM; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF GLYCEROL; RESIN SOAPS; SOAP OR SOAP-MAKING; SPIRITS; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

본 발명은 호알칼리성 Bacillus sp.의 균주로부터 추출된 세정제 프로테아제의 분야에 속한다. 더 특이적으로는, 본 발명은 Bacillus sp. JP395 균주로부터 추출된 프로테아제에 관한 것이다. 더욱 본 발명은 프로테아제의 제조방법, 세정제 효소로서 프로테아제의 이용, 및 본 발명의 프로테아제를 포함하는 세정제 조성물에 관한 것이다. PCT No. PCT/DK92/00105 Sec. 371 Date Sep. 23, 1993 Sec. 102(e) Date Sep. 23, 1993 PCT Filed Apr. 3, 1992 PCT Pub. No. WO92/17578 PCT Pub. Date Oct. 15, 1992.An alkaline protease from a strain of Bacillus sp. JP 395 has been isolated. The protease has a mass of about 30 kDa as determined by SDS-PAGE, a pH optimum of about 9-11, a temperature optimum of about 50 DEG -65 DEG C., and an isoelectric point above 9.5. The protease is inhibited by PMSF (phenylmethylsulfonylfluoride), but not by EDTA or soybean protein inhibitor. The protease is prepared by growing this strain of bacillus in a suitable media and recovering the protease. The protease has been formulated in detergents and is stable at 40 DEG C. for 60 minutes in these detergents under wash conditions in the presence or absence of bleaches such as perborate.