PHOSPHOTYROSINE ASSAY AND PEPTIDES FOR USE THEREIN

• Main Authors: WILKS, ANDREW FREDERICK, OATES, ANDREW CHARLES, LACKMANN, MARTIN, HARPUR, AILSA GWENDOLINE
• Format: Patent
• Language: English; Korean
• Published: 01.02.2000
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

본 발명은 시토킨 리셉터 세포내 펩티드와 상호작용할수 있는 분자의 분석과 상기 분석에서 사용하기에 적당한 펩티드에 관한 것이다. 본 펩티드는 시토킨 호르몬 리셉터 또는 JAK 단백질 티로신 키나아제의 세포내 도메인 서열의 티로신-함유 부위에 해당하며, 인산화될수 있는 티로신을 함유한다. 인산화된 형태에서, 본 펩티드는 STAT 패밀리의 세포질 전사 인자에 결합할수 있다. This invention relates to an assay for molecules capable of interacting with cytokine receptor intracellular peptides, and to peptides suitable for use in said assay. The peptides correspond to tyrosine-containing regions of the sequence of an intracellular domain of a cytokine, a hormone receptor or a JAK Protein Tyrosine Kinase, and comprise a tyrosine which is able to be phosphorylated. In phosphorylated form, the peptides are able to bind cytoplasmic transcription factors of the STAT family.