MELANIN PRODUCTION BY TRANSFORMED MICROORGANISMS

• Main Authors: TURPEN, THOMAS H, GARGER JR., STEPHEN J, CHEDEKEL, MILES R, SVERLOW, GENADIE G, DELLA-CIOPA, GUY, GRILL, LAURENCE K, KUMAGAI, MONTO H
• Format: Patent
• Language: English; Korean
• Published: 17.08.1998
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

본 발명은 멜라닌의 제조방법, 활성디로시나제의 제조방법에 관한 것으로써, 본 발명에 따른 멜라닌제조방법은 멜라닌제조효소를 코딩하는 제1DNA씨퀀스와 활성제를 코딩하는 제2DNA씨퀀스인, 멜라닌을 생성하는데 유용한 둘이상의 DNA씨퀀스를 함유하도록 미생물을 형질전환시켜서 멜라닌을 생성하도록 되어있고, 상기 제1DNA씨퀀스가 티로시나제를 코딩하며, 이것은 진균, 세균, 인간, 동물 또는 식물에서 얻도록 되어 있다. 또한 본 발명에 따른 활성티로시나제의 제조방법은 E. Coli세포를 티로신을 코딩하는 씨퀀스를 함유하는 DNA단편과 활성제단백질을 코딩하는 씨퀀스를 함유하는 DNA단편에 형질전환시키고, 형질전환된 E. Coli세포를 적당한 배지에서 배양하는 것으로 되어 있다. The present invention is directed to a process for producing melanins, their precursors and their analogs, hereinafter referred to generically as melanins. According to the invention, melanins are produced in amounts greater than about 0.2 grams dry weight per liter of growth medium. The enhanced production of melanin can be achieved by manipulating the constituents of the growth medium, and/or attenuating fermentation conditions, and/or by genetically engineering microorganism to produce melanins, and/or mutating the microorganisms.