MATERIALS AND METHOD FOR BIOSYNTHESIS OF SERINE AND SERINE-RELATED PRODUCTS

• Main Author: BURLINGAME, RICHARD,P
• Format: Patent
• Language: English; Korean
• Published: 11.04.1998
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

야생형 PGD와 비교하여 세린(Serine)에 의한 저해에 대한 감수성을 감소시킨 3-포스포글리세린산염 탈수소효소(PGD)를 엔코딩하는 DNA, 즉 DNA는 적어도 생합성에 유용한 효소활성을 가지며, (변성없는) 야생형 PGD보다 더 높은 레벨의 세린에서의 효소활성을 보유하는 PGD를 엔코딩한다. 이 발명은 또 a) 위 공학적 DNA의 아미노산 배열을 가진 PGD와, b) 공학적 DNA와 숙주발현계에서 그 공학적 DNA의 발현을 하도록 배치하여 지향한 조절 DNA를 포함하는 발현벡터와, c) 이와같은 발현벡터를 포함하는 세포와, d) 이와같은 세포를 배양시켜 세린 또는 세린 유도생성물을 제조하는 방법에 대한 것이다. PCT No. PCT/EP92/01873 Sec. 371 Date May 24, 1994 Sec. 102(e) Date May 24, 1994 PCT Filed Aug. 17, 1992 PCT Pub. No. WO93/12235 PCT Pub. Date Jun. 24, 1993Engineered DNA encoding 3-phosphoglycerate dehydrogenate (PGD) has reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. The PGD has the amino acid sequence of this above-described engineered DNA. Expression vectors contain this engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system. Cells contain these expression vectors, and a method for producing serine or a serine-derived product includes culturing such cells.