DNA CHAIN

PURPOSE:To obtain a DNA chain containing a biosynthetic gene for a carbapenem based antibiotic substance, by digesting the whole chromosomic DNA of Streptomyces.fulvoviridis with a restriction enzyme PstI. CONSTITUTION:The aimed DNA fragment of about 6.0kb mol. wt. obtained by digesting chromosomic...

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Bibliographic Details
Main Authors UOZUMI TAKESHI, NAKADA KUNIO, BEPPU TERUHIKO, HORINOUCHI SUEJI
Format Patent
LanguageEnglish
Published 15.02.1989
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE:To obtain a DNA chain containing a biosynthetic gene for a carbapenem based antibiotic substance, by digesting the whole chromosomic DNA of Streptomyces.fulvoviridis with a restriction enzyme PstI. CONSTITUTION:The aimed DNA fragment of about 6.0kb mol. wt. obtained by digesting chromosomic DNA of Streptomyces.fulvoviridis N1501 with PstI, subjecting the resultant DNA fragment with a plasmid PIJ385 similarly treated with PstI to ligation treatment using a T4 ligase, introducing the ligation product into an FN24 strain, selecting a plasmid pCPG31 incorporating a biosynthetic gene of a carbapenem based antibiotic substance in a selection culture medium and then treating the above-mentioned plasmid with the PstI and having one recognition site with BglII, KpnI and SacI and tow recognition sites with BamHI and XhoI. Furthermore, a DNA fragment of about 1.7kb prepared by digesting the afore-mentioned DNA fragment with BamHI is also capable of imparting the ability to produce an antibiotic substance to a microorganism, such as FN24 or FN27, incapable of producing the antibiotic substance.
Bibliography:Application Number: JP19870199131