SEPARATING AND RECOVERING METHOD OF CELLULOSE PRODUCED BY MICROORGANISM

• Main Authors: SAKA SHIRO, YAMASHITA HARUHIKO
• Format: Patent
• Language: English
• Published: 26.03.1988
• Subjects: APPARATUS THEREFOR; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; PAPER-MAKING; POLYSACCHARIDES; PROCESSES USING MICROORGANISMS; PRODUCTION OF CELLULOSE; PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; SPIRITS; TEXTILES; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

PURPOSE:To separate and recover a cellulose produced by a bacterium capable of producing the cellulose from a culture medium in high purity and yield, by subjecting an mixture containing a bacterial cell capable of producing the cellulose and the cellulose to alkali treatment, ultrasonic treatment and then centrifugation. CONSTITUTION:A mixture containing a bacterium capable of producing a cellulose and the cellulose is treated by an alkali and then ultrasonic waves and successively centrifuged. In the above-mentioned alkali treatment, and aqueous solution of alkali such as sodium hydroxide, potassium hydroxide, etc., is used in a concentration of 0.01-15wt%, preferably about 1wt%. If the alkali concentration is <0.01wt%, it is difficult to sufficiently break the bacterial cell and if the concentration is >15wt%, there is the possibility of causing depolymerization of the cellulose and structural change from cellulose I to cellulose II. The alkali treatment is carried out preferably at nearly ordinary temperature. As the ultrasonic waves, elastic waves whose frequency exceeds audible frequency area, preferably waves having >=10kHz frequency are used.