NOVEL ALDEHYDE DEHYDROGENASE COMPLEX AND PRODUCTION THEREOF

NEW MATERIAL:An aldehyde dehydrogenase complex having the following characteristics. (A) An enzyme complex containing proteins having molecular weights of about 75,000 and about 20,000 determined by SDS-polyacrylamide electrophoresis, (B) containing 4,5-dihydro-4,5-dioxo-1H-pyrrolo[2,3- f]quinoline-...

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Main Authors TAYAMA KENJI, FUKAYA MASAHIRO, KAWAMURA KICHIYA, TAGAMI ATSUKO, OKUMURA HAJIME
Format Patent
LanguageEnglish
Published 19.01.1988
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:NEW MATERIAL:An aldehyde dehydrogenase complex having the following characteristics. (A) An enzyme complex containing proteins having molecular weights of about 75,000 and about 20,000 determined by SDS-polyacrylamide electrophoresis, (B) containing 4,5-dihydro-4,5-dioxo-1H-pyrrolo[2,3- f]quinoline-2,7,9-tricarboxylic acid (C) capable of oxidizing >=2C aldehyde as substrate, (D) solubilizable from cytoplasm membrane with a surfactant and (E) exhibiting residual activity of >=80% even after being left for 1 week at 3-10pH and 4 deg.C. USE:Determination of aldehyde in brewed food or during fermentation of Japanese rice wine. PREPARATION:A microbial strain belonging to Acetobacter genus such as Acetobacter altoacetigenes MH-24 (FERM BP-491) is cultured and the objective substance is separated from the cultured product.
Bibliography:Application Number: JP19860156143