NOVEL MICROORGANISM

PURPOSE:To obtain a novel microorganism, capable of converting a diester into a half ester and belonging to the genus Bacillus, by cultivating and separating Bacillus sp. VA-47 strain (FERM-P No.8730) from soil. CONSTITUTION:A novel microorganism obtained by adding, e.g. supernatant liquid of a susp...

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Bibliographic Details
Main Authors KUMAGAI KAZUO, YASUKUI HIDEO, FUKITA YOSHIKAZU, TAYA KIYOSHI
Format Patent
LanguageEnglish
Published 20.10.1987
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE:To obtain a novel microorganism, capable of converting a diester into a half ester and belonging to the genus Bacillus, by cultivating and separating Bacillus sp. VA-47 strain (FERM-P No.8730) from soil. CONSTITUTION:A novel microorganism obtained by adding, e.g. supernatant liquid of a suspension of sterilized physiological saline solution of soil to a separating culture medium, carrying out shaking cultivation, smearing a separating agar culture medium with the culture to carry out cultivation, repeating separation of the formed colonies with a separating agar culture medium to obtain completely purified Bacillus sp. VA-47 strain. The VA-47 strain has an ability to convert cis-imidazolidinedicarboxylic acid diester into an optically active imidazolidinedicarboxylic acid derivative (half ester) by sultivating in the presence of the diester. Various culture media containing a carbon source, nitrogen source, inorganic substances, etc., may be used as the culture medium for the above-mentioned VA-47 strain.
Bibliography:Application Number: JP19860082484