IMMUNOLOGICAL MEASUREMENT METHOD FOR BACILLUS RETAINED IN BACTERIAL EXOSPORIUM

• Main Authors: HOSHINO HIDEAKI, HORIGOME KAZUMI
• Format: Patent
• Language: English
• Published: 02.07.1987
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

PURPOSE:To measure all influenza bacilli without overlooking regardless of types and to improve the degree of detection by bringing the antibody obtd. by immunizing the crude exosporium protein of the hemophilus influenza bacillus as an antigen into an animal into an immunological reaction with a specimen. CONSTITUTION:Any influenza bacilli are usable regardless of the types thereof. A specified amt. of the crude exosporium protein is inoculated intracutaneously, subcutaneously, intramuscularly, intraperitoneally and intraveneously to the animal or into the sole of a foot thereof several times, by which the antiserum is obtd. The immunogical reaction which is generally practised may be simply executed in the case of measuring the influenza bacilli in the specimen by using the antiserum (antibody) prepd. in the above-mentioned manner; for example, an enzyme immunoassay, radioimmunoassay, direct fluorescent antibody method, indirect fluorescent antibody method, reverse passive hemagglutination method, reverse passive latex agglutination method and coagulation method are usable.