METHOD FOR SEPARATING GENE OF ACETIC ACID BACTERIA

PURPOSE:To separate rapidly and readily genes of acetic acid bacteria, by expressing the genes of acetic acid bacteria utilizing the gene expression ability of the genes of acetic acid bacteria in Escherichia coli using an Escherichia coli host vector system and separating the resultant genes of ace...

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Main Authors TAYAMA KENJI, FUKAYA MASAHIRO, TAGAMI HARUKO, OKUMURA HAJIME, MASAI HIROSHI
Format Patent
LanguageEnglish
Published 12.12.1986
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE:To separate rapidly and readily genes of acetic acid bacteria, by expressing the genes of acetic acid bacteria utilizing the gene expression ability of the genes of acetic acid bacteria in Escherichia coli using an Escherichia coli host vector system and separating the resultant genes of acetic acid bacteria. CONSTITUTION:Genes of acetic acid bacteria are expressed using an Escherichia coli host vector system, e.g. Escherichia coli C600, Escherichia coli HB101 or Escherichia coli LE392, utilizing the gene expression ability of the genes of acetic acid bacteria in the Escherichia coli and then separated. Bacteria belonging to the genus Acetobacter, Gluconobacter or Frateuria are used as the acetic acid bacteria. Genes thereof include those produced from Acetobacter aceti (FERM-P No.7122). According to this method, the aimed useful genes of acetic acid bacteria can be rapidly and readily separated.
Bibliography:Application Number: JP19850122673