PARTICLE ANALYZING INSTRUMENT

PURPOSE:To execute fluorometry with high accuracy by passing the fluorescence from a specimen which scatters the light irradiated thereon to a lateral side approximately with the advancing direction of said light through a spectral means such as prism or diffraction grating, etc. and detecting the l...

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Bibliographic Details
Main Author KAWAGUCHI BUNRO
Format Patent
LanguageEnglish
Published 04.08.1986
Edition4
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:PURPOSE:To execute fluorometry with high accuracy by passing the fluorescence from a specimen which scatters the light irradiated thereon to a lateral side approximately with the advancing direction of said light through a spectral means such as prism or diffraction grating, etc. and detecting the light by a line sensor, etc. CONSTITUTION:The specimen such as hemocyte cells enveloped by a sealing liquid and labeled by a fluorescent agent is passed through the inside of a passage part 2 at the center of a cell 1 in a particle analyzing instrument to be used for a flow sight meter, etc. The fluorescence to be emitted to the lateral side approximately orthogonal with the advancing direction of the irradiated light L is made into a parallel luminous flux by a collimator lens 8. Said flux is divided to continuous frequency components by a spectral prism 9 and the components are condensed by a lens 10 and are made incident to a photoelectric detector 11 such as line sensor. The output thereof is processed by an arithmetic means and the information for analyzing the sample S is obtd. The photometry is thus made possible with high wavelength resolution without requiring an intricate optical system.
Bibliography:Application Number: JP19850013736