JPS6116280B

• Main Authors: AZUMABASHI NOBUYUKI, KONO MORIHIRO, SHIBATA SHOJI, MIKAWA USHIO, KANDA NOBUO, SATO KYOSHI, ABE SHIGERU, TAKAHASHI KUNIO, YAMAZAKI MASATOSHI, MIZUNO DENICHI
• Format: Patent
• Language: English
• Published: 28.04.1986
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOUNDS OF UNKNOWN CONSTITUTION; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; PEPTIDES; POLYSACCHARIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

NEW MATERIAL:An antitumor protein polyglycoside DH-6665 comprising D-glucose (G) and D-mannose (M) in the glucide moiety at a ratio of (G) to (M) of 2:1, and glycine, alanine, glutamic acid, and dicyanopimelic acid in the protein moiety, readily soluble in water, soluble in formamide, and slightly soluble in DMSO. Elementary analysis: C, 35-43%; H, 4.8-6.4%; N, 0.5-2.5%, free from halogen and S. Molecular weight: 10,000-300,000. Specific rotatory power: [alpha]D = +30-+70 deg.. USE:Antitumor agents capable of promoting the immunity of hosts. PROCESS:Microellobosporia grisea strains (IFO-12518) are cultured in a liquid medium at 28-37 deg.C, and the objective substance is collected from the filtrate, and purified by gel filtration to give the antitumor protein polyglycoside DH-6665.