DIAGNOSING/EXAMINATION AGENT

• Main Authors: NAGAIKE KAZUHIRO, MURAMATSU MINORU, HOSOKAWA SEIKO
• Format: Patent
• Language: English
• Published: 18.04.1985
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

PURPOSE:To enable the diagnosing and examination of cancer cells by using a monoclonal antibody for recognizing alpha-fetoprotein existing on the surface of a cell membrane. CONSTITUTION:A monoclonal antibody for recognizing alpha-fetoprotein (AFP) as marker of cancer existing on the membrane surface of a cancer cell used to be a diagnosing/inspecting agent for cancers. To obtain the monoclonal antibody, human AFP is immunized to a mouse. Thereafter, the spleen is extracted and fused with mouse's myeloma cell using polyethylene glycol to obtain a hydridoma. Then, a monoclonal antibody is obtained from the supernate. The antibodies thus obtained are used to dye a liver cancer cell line and an antibody indicating a positive value is selected. The massproduction of monoclonal antibodies can be done by cultivating a large amount of hybridoma in a cultivation tank.