MONOCLONAL ANTIBODY

• Main Authors: NAGAIKE KAZUHIRO, MURAMATSU MINORU, HOSOKAWA SEIKO
• Format: Patent
• Language: English
• Published: 17.04.1985
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

PURPOSE:To provide a monoclonal antibody recognizing the alpha-fetoprotein existing at the surface of cell membrane, and useful for the remedy of hepatocarcinoma, especially as an antibody for missile therapy. CONSTITUTION:A monoclonal antibody recognizing the alpha-fetoprotein (AFP) exsisting at the surface of cell membrane. In the markers of carcinoma, AFP and fetus antigen (CEA) are well known, and AFP is produced in various hepatocarcinoma and cerulein. Since said monoclonal antibody recognizes the AFP existing at the surface of cell membrane, it is suitable for the missile therapy by bonding the antibody with a carcinostatic agent or a toxin. The antibody can be prepared by (1) immunizing e.g. BALB/C mouse with AFP originated from human placenta, (2) extracting the spleen from the immunized animal, (3) carrying out the fusion with the mouse myeloma cell of e.g. P3-U1 using polyethylene glycol by conventional method to obtain a hybridoma, and (4) separating from the supernatant liquid. The monoclonal antibody prepared by this process is effective to dye the hepatocarcinoma cerulein immunochemically, and to select the positive antibody.