NOVEL ENHANCING FACTOR FOR PREPARATION OF HUMAN ANTIBODY AND ITS PREPARATION

• Main Authors: HONGOU TERUAKI, YOSHIDA TAKAHITO, KOIDE YUKIO, HARAGUCHI SOUICHI
• Format: Patent
• Language: English
• Published: 14.02.1985
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

PURPOSE:To obtain a novel enhancing factor for preparation of human antibody, by subjecting a human lymphocyte attached to nylon wool to isolation culture, dialyzing the culture solution, and lyophilizing the filtrate through the dialysis membrane, if necessary. CONSTITUTION:An enhancing factor for preparation of human antibody, having 2,000-4,000mol.wt., strengthening and promoting preparation of human antibody obtained by isolation culture from human lymphocyte cell. The factor, for example, is obtained by preparing a mononuclear cell from human amygdala, separating lymphocyte from the mononuclear cell, suspending the prepared lymphocyte in a culture solution containing bovine fetal serum, blending the suspension with nylon wool, eluting the blend with the same culture solution, to give a fraction of lymphocyte attached to the nylon wool. The attached lymphocyte is washed out from the nylon wool, centrifuged, the lypphocyte is suspended in a culture solution containing bovine fatal serum, cultivated in a carbonic acid gas flow at 35-38 deg.C for 1-7 days, the culture is centrifuged, the lymphocyte is removed, the supernatant liquid is passed through a dialysis membrane, and the filtrate is lyophilized to give the desired factor.