NOVEL SYNTHESIS SUBSTRATE

• Main Authors: MATSUMOTO KUNIO, OOTSUKA AKIRA, KAGIMOTO YOSHITAKA, WATANABE SUSUMU, TAKAHASHI SEIJI
• Format: Patent
• Language: English
• Published: 09.04.1983
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; BEER; BIOCHEMISTRY; CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

NEW MATERIAL:A compound shown by the formulaI(X1 is bond group or alkylene which may have substituent group; X is phenyl, substituted phenyl, lower alkyl, -COOH, -SO3H, or lower alkylthio) or its salt. EXAMPLE:N-L-Leucyl-phenetylamide. USE:A substrate for measuring the activity of LAP. A substituted methylamine compound which is liberated by the action of LAP is reacted with a corresponding oxidase, and the amount of oxygen used or hydrogen peroxide formed by the reaction is measured. PROCESS:An L-leucine shown by the formula II is reacted with a substituted methylamine compound shown by the formula III by condensation reaction (e.g., carbodiimide method, azido method) useful for peptide synthesis, to give a compound shown by the formulaI. If necessary, the reaction is carried out after the protection of functional groups, and the protecting groups are removed after the condensation.