REGENERATION OF IMMOBILIZED YEAST

• Main Authors: SAZANAMI TSUNEO, WATANABE KEIICHIROU, YAMADA TOMIAKI, TAKIGUCHI KOUJI
• Format: Patent
• Language: English
• Published: 11.07.1983
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PURPOSE:To regenerate the activity of immobilized yeast for the alcoholic fermentation of sugar solution, by treating the yeast with an aqueous solution of a phosphate, thereby dissolving and removing the precipitate deposited to the surface of the immobilized yeast. CONSTITUTION:An aqueous solution of one or more compounds selected from secondary orthophosphates (e.g. secondary sodium phosphate), tertiary orthophosphates (e.g. tertiary potassium phosphate), and condensed phosphates (e.g. sodium tripolyphosphate), is used as the treating agent. When the alcoholic fermentation is carried out continuously by contacting an immobilized yeast with a sugar solution, the activity of the yeast decreases gradually by the deposition of the solid suspension in the solution to the surface of the yeast. The surface deposit can be removed by discharging the sugar solution from the reaction system, charging the above aqueous solution of phosphate (0.1-10wt% concentration, 3-10pH) in the system and reacting with the immobilized enzyme at <=45 deg.C.