METHOD AND KIT FOR PREPARING SAMPLE USED FOR TESTING ENDOTOXIN WITH WATER-INSOLUBLE ANTIHOMO ALPHA2-MACROGLOBULIN ANTIBODY

• Main Authors: KAWAKADO KEIICHI, SHIYOUJI TAKASHI, MAEDA SHIYOUICHI, WATANABE RIYOUZOU
• Format: Patent
• Language: English
• Published: 04.08.1982
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

PURPOSE:To prepare samples used for testing endotoxin without including any analysis disturbing component by a method wherein samples of blood plasma or blood producing agents are developed through a column filled with an antihomo alpha2-macroglobulin antibody which has become water-insoluble with crosslinked aryldextran. CONSTITUTION:A water-insoluble antibody 2 of 100-300 mesh, which is obtained by chemically combine an antihomo alpha2-macroglobulin antibody with aryldextran crosslinked with N,N'-methylenebis-acrylamido after having been treated with BrCN or so, is filled in a column 1 with an inner diameter of 7-12mm. by an amount of 2-4ml and then a tight stopper 4 is fitted. The above solidified antibody 2 is placed between a support plate 6 and a holding plate 5 in the column 1, while a lower buffer solution layer 8 is enclosed by a bottom cover 7 and supernatant fluid 3 also comprises buffer solution. Meanwhile, an ampul of water solution containing high molecular coloring materials to be added to sample and an ampul of buffer solution for developing are readied, so that a preparing kit is obtained as a set. Samples such as blood plasma are mixed with the above water solution containing the coloring materials and caused to flow down through the column, thereby to develop the sample with the developing solution. The eluted colored fractions are collected and then used as samples for testing.