GENE TRANSFER THROUGH ELECTROPORATION

PROBLEM TO BE SOLVED: To directly transfer a foreign gene into a plant tissue fragment or a callus without making it protoplasmic and with simplicity and rapidness by conducting electroporation under a specific condition. SOLUTION: The method of this invention is to transfer a foreign gene into a pl...

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Bibliographic Details
Main Authors KANZAKI HIROYUKI, EI HITOSHI
Format Patent
LanguageEnglish
Published 02.03.1999
Edition6
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To directly transfer a foreign gene into a plant tissue fragment or a callus without making it protoplasmic and with simplicity and rapidness by conducting electroporation under a specific condition. SOLUTION: The method of this invention is to transfer a foreign gene into a plant tissue fragment or a callus through electroporation where the electrical field is 50-500 V/cm, the length of the electric pulse is 30-200 msec and the impressing number of times is 5-25 times. It is preferable to immerse the plant tissue fragment or the callus into solution containing an osmotic pressure regulating agent and/or an intercellular dissociation enzyme for 15 min. to 5 hrs. before the electroporation.
Bibliography:Application Number: JP19970230666