SUGAR CHAIN-BRANCHED COMPOSITIONAL MODIFICATION INTERFERON GAMMA

• Main Authors: TANIGAWA MINEKO, MAKINO TADASHI, FUKUDA KAZUHIRO, MINOWA MARI, BIZEN FUMIO, TAKEUCHI MAKOTO
• Format: Patent
• Language: English
• Published: 21.12.1999
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To obtain the subject interferon γ with enzymatic activity maintained at high levels, excellent in medicinal sustainability, and useful as a medicament or the like by culturing specific transformed cells in a medium. SOLUTION: This sugar chain-branched compositional modification interferon γis obtained by transferring an N-acetylglucosaminyl transferase IV gene and/or an N-acetylglucosaminyl transferase V gene into animal host cells such as a CHO strain capable of producing human interferon γ to afford transformed cells, which, in turn, are cultured in a medium under aerobic conditions. By the way, it is preferable that pharmaceutical preparations are manufactured by including the interferon γ as active ingredient.