GENES CODING FOR DESULFURIZING ENZYMES

PROBLEM TO BE SOLVED: To obtain a new gene which has a specific amino acid sequence, converts dibenzothiophenesulfonic acid to 2-(2'-hydroxyphenyl)benzenesulfinic acid, and can be used, for example, for producing an enzyme capable of easily releasing sulfur in the fossil fuel. SOLUTION: This is...

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Bibliographic Details
Main Authors HIRASAWA KAZUAKI, ISHII YOSHITAKA, KONISHI HITOSHI, OKADA HIDEKI, SUZUKI MASANORI
Format Patent
LanguageEnglish
Published 14.12.1999
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CRACKING HYDROCARBON OILS
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
FUELS
LUBRICANTS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MINERAL WAXES
MUTATION OR GENETIC ENGINEERING
PEAT
PETROLEUM, GAS OR COKE INDUSTRIES
PROCESSES USING MICROORGANISMS
PRODUCTION OF LIQUID HYDROCARBON MIXTURES, e.g. BY DESTRUCTIVEHYDROGENATION, OLIGOMERISATION, POLYMERISATION
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
RECOVERY OF HYDROCARBON OILS FROM OIL-SHALE, OIL-SAND, ORGASES
REFINING MIXTURES MAINLY CONSISTING OF HYDROCARBONS
REFORMING OF NAPHTHA
SPIRITS
TECHNICAL GASES CONTAINING CARBON MONOXIDE
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To obtain a new gene which has a specific amino acid sequence, converts dibenzothiophenesulfonic acid to 2-(2'-hydroxyphenyl)benzenesulfinic acid, and can be used, for example, for producing an enzyme capable of easily releasing sulfur in the fossil fuel. SOLUTION: This is a new gene which codes for a protein having an amino acid sequence shown by the formula, or an amino acid sequence which one or more amino acid(s) is/are deleted from, substituted in, or inserted into the amino acid sequence shown by the formula, and has a function of converting dibenzothiophenesulfonic acid to 2-(2'hydroxyphenyl)benzenesulfinic acid, and is useful, for example, for producing a desulfurizing enzyme capable of easily releasing sulfur in the fossil fuel. This gene is obtained by preparing the whole DNA library from a thermophilic desulfurizing bacterium Paenibacillus sp. strain A11-2, followed by screening the library using a labelled partial gene of the desulfurizing enzyme as a prove.
Bibliography:Application Number: JP19980310545