PROTEIN AS CONSTITUENT OF HUMAN 26S PROTEASOME AND DNA ENCODING THE SAME

• Main Authors: SEKINE SHINGO, KATO MASASHI
• Format: Patent
• Language: English
• Published: 09.02.1999
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To obtain the subject new protein, composed of human 26S proteasome constituent protein having a specific amino acid sequence, capable of exhibiting an important role in an intracellular proteolysis system, and useful for, e.g. elucidation of proteasome functions, or diagnosis or treatment of malignant tumor or the like. SOLUTION: This protein is composed of human 26S proteasome constituent protein having a specific amino acid sequence shown by the formula, capable of exhibiting an important role in an intracellular proteolysis system, and useful for, e.g. elucidation of proteasome functions, or diagnosis or treatment of diseases, e.g. malign lump, in which proteasome is involved. The protein is obtained by (A) synthesizing cDNA using a mold of poly (A)<+> RNA extracted from human cells, (B) preparing a library of cDNA derived from the human cells by the normal procedure using the above cDNA, and (C) genetically recombining a gene, obtained by screening using an oligonucleotide probe composed of partial, basic sequence of bovine 26S proteasome constituent protein, into a expression vector, and expressing it in a host cell, e.g. that of E coli.