CONTROLLING OF SPERMATOGENESIS USING MEA-2 GENE INFORMATION

• Main Authors: KONDO MASAAKI, SUDOU SHIZUYO, MATSUKUMA SHOICHI
• Format: Patent
• Language: English
• Published: 26.01.1999
• Edition: 6
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for producing organisms of controlled spermatogenesis useful for test animals or the like, capable of controlling spermatogenesis, producing sterilized male animals and providing an index for spermatogenesis-related diseases by damaging Mea-2 gene totally or partially. SOLUTION: This method damages, totally or partially, Mea-2 gene containing a base sequence shown by the formula or the one hybridizable with the above, and also an intron. It is preferable that the damaged section in the Mea-2 gene is in the boundary between the intron and exon, and has a size of 1 bp to 1 Kbp. It is also preferable that the male organism (except human), controlled in spermatogenesis by totally or partially damaging its Mea-2 gene, is transplanted with a spermatogonia of the similar or dissimilar species having a target gene, e.g. a gene capable of coding a physiologically active material, to make it possible to form the spermatogonia-derived sperm in the individium having the damaged Mea-2 gene and to breed the organism having a target gene by mating the above male individium with a female individium.