MICROPROPAGATION OF PLANT BELONGING TO GENUS DISA

• Main Authors: JO SHINICHIRO, TSUTSUI KIYOSHI
• Format: Patent
• Language: English
• Published: 26.01.1999
• Edition: 6
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To micropropagate the subject plant for mass-producing the clone seedling of the subject plant belonging to the family Orchidaceae, by sterilely taking out an ovary or its neighborhood part from a flower bud and culturing a medium containing a phytohormone. SOLUTION: A flower bud completely wrapped up in straw on the upper part of a spike is collected after the first flowering period of a plant of the genus Disa, the surface of the flower bud is sterilized, an ovary or its neighborhood part is sterilely taken out, longitudinally divided and cultured in a medium containing a phytohormone. The surface sterilization of the collected flower bud can be carried out by spraying 70% alcohol to the whole surface, after drying the surface, immersing the flower bud in a chlorine-based germicide such as sodium hypochlorite having 1% effective chlorine, containing about 0.01% of a surfactant such as Tween 20 for about 10 minutes while shaking. A combination of an auxin and a cytokinin can be used as the phytohormone.