POLYGALACTURONASE AND ITS PRODUCTION

PROBLEM TO BE SOLVED: To provide polygalacturonase having glucose resistance, and to provide a method for producing the same. SOLUTION: This polygalacturonase is produced with a microorganism belonging to the genus Cryptococcus, and has the following physicochemical properties (1)-(9): (1) hydrolyze...

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Bibliographic Details
Main Authors SAKAMOTO YASUTERU, SATO MITSUKATSU, YANAI TAKAAKI
Format Patent
LanguageEnglish
Published 29.06.1999
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To provide polygalacturonase having glucose resistance, and to provide a method for producing the same. SOLUTION: This polygalacturonase is produced with a microorganism belonging to the genus Cryptococcus, and has the following physicochemical properties (1)-(9): (1) hydrolyzes the α-1,4,-bond of poly-D-galacturonic acid; (2) has the optimal pH of 3.5; (3) exhibits a stable enzymatic activity at a pH of 3.0-6.0, when held at 20 deg.C for 6 hr; (4) has the optimal temperature of about 22 deg.C; (5) exhibits a stable enzymatic activity of up to 25 deg.Cm, when held at a pH of 4.2 for 3 hr, and is gradually deactivated above the pH; (6) is not inhibited by mercuric chloride but scarcely by ethylenediaminetetra-acelic acid; (7) has a mol.wt. of about 70,000 dalton measured by a SDS-polyacrylamide gel electrophoresis method, (8) has an isoelectric point at a pH of 14.8, and (9) exhibits a relative activity of about 80% in a 500 mM glucose solution.
Bibliography:Application Number: JP19970362367