DETECTION OF PROTEASE ACTIVITY

• Main Authors: KAGIMOTO YOSHITAKA, YOSHINARI KAHORI
• Format: Patent
• Language: English
• Published: 27.04.1999
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for detecting the subject activity, e.g. enzyme activity and hemolytic activity, in a highly sensitive way, by treating a specimen with a pure substrate highly sensitive to protease and having detectable biological activity, then, detecting changes in the biological activity of the substrate. SOLUTION: This method comprises treating a specimen with a practically pure substrate highly sensitive to protease and having an enzyme activity, e.g. glycerol-3-phosphate oxidase activity or ascorbate oxidase activity or hemolytic activity, e.g. streptolysin O hemolytic activity as a detectable biological activity, then, detecting changes in the biological activity of the substrate and thereby, detecting the objective protease activity usable for detecting, e.g. proteases in a specimen, e.g. biological components and, contaminant protease in the specimen.