COLLECTING METHOD OF HEMATOPOIETIC STEM CELL, COLLECTING APPARATUS AND COLLECTING DEVICE

• Main Authors: TAKAHASHI TSUNEO, YASUTAKE KANCHI
• Format: Patent
• Language: English
• Published: 07.04.1998
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To collect hematopoietic stem cells and to easily decrease blood cell components except for the hematopoietic stem cells to leave the plasma protein component by introducing a suspension liquid of hematopoietic stem cells into a sampling device for hematopoietic stem cells having a specified structure and then treating the liquid under specified conditions. SOLUTION: A collecting apparatus 7 for hematopoietic stem cells is prepared by packing a container 3 having an inlet 1 and outlet 2 for a suspension liquid of hematopoietic stem cells with a fractionating layer 4 as a main component. This layer 4 consists of one of porous material, fiber aggregates and particle aggregates and has 1 to 60μm average pore diameter. A suspension liquid of hematopoietic stem cells is introduced into the collecting apparatus 7 and the liquid is made to substantially flow upward in a gravity field. A plasma which is first drained from the collecting apparatus 7 for hematopoietic stem cells is collected. Then red blood cells and blood platelets are drained and a cell aggregate containing hematopoietic stem cells is collected.