IDENTIFICATION OF ODORANT, NEW DNA FOR THE SAME, RECOMBINANT VECTOR CONTAINING THE DNA, MICROORGANISM CONTAINING THE VECTOR, ODOR SENSOR COMPRISING THE MICROORGANISM AND DETECTION OF 2-METHYLISOBORNEOL BY USING THE MICROORGANISM

• Main Authors: NISHINO TOKUZO, ONUMA SHINICHI, OIKAWA EISAKU
• Format: Patent
• Language: English
• Published: 24.02.1998
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for identifying an odorant by using a specific microorganism manifesting a detectable signal when the microorganism is brought into contact with the odorant, and to obtain a new DNA having a variation of a CamR gene coding a specific amino acid sequence capable of producing the microorganism useful as an odor sensor capable of specifically detecting 2-methylisoborneol and used for identification of a material having a musty smell in tap water. SOLUTION: An odorant, e.g. 2-methylisoborneol is detected in high sensitivity from a ratio of quantities of signals obtained by bringing a microorganism capable of manifesting a signal by a contact with the odorant and giving different quantities of the signals against the same material at the different first and second temperatures into contact with a sample to be detected at the first and the second temperatures in the method for identifying the odorant. The microorganism includes a new DNA obtained by treating a DNA fragment containing a repressor gene in a camphor operon (CamR) coding an amino acid sequence of the formula with a mutagen, which can detect 2-methylisoborneol in a much higher sensitivity compared to a wild type strain or other variants and including a recombinant vector having an operator/promoter region in the campher operon and a reporter gene.