NEW PROTEIN HAVING ASPARTASE ACTIVITY AND GENETIC DNA ENCODING THE SAME

PROBLEM TO BE SOLVED: To obtain the subject new protein consisting of a protein having a specific amino acid sequence, to be used for producing L-aspartic acid useful as e.g. a medicine, food additive, surfactant, raw material for biodegradable chelating agents. SOLUTION: This new protein, which has...

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Bibliographic Details
Main Authors TO FUJIO, WATANABE FUMIAKI
Format Patent
LanguageEnglish
Published 22.12.1998
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To obtain the subject new protein consisting of a protein having a specific amino acid sequence, to be used for producing L-aspartic acid useful as e.g. a medicine, food additive, surfactant, raw material for biodegradable chelating agents. SOLUTION: This new protein, which has aspartase activity, has an amino acid sequence of the formula where, optionally, amino acid(s) are deleted, substituted or added, and is used for producing L-aspartic acid useful as e.g. a medicine, food additive, surfactant, raw material for biodegradable chelating agents from fumaric acid and ammonia. This new protein is obtained by the following process: a DNA is isolated from Rhodococcus sp. EA4 (FERM P-12136), a DNA library is made using the above DNA by conventional means, and then screened using an aspartase gene fragment as probe, and the resulting aspartase gene is integrated into a vector and expressed in host cells.
Bibliography:Application Number: JP19970163538