TEST OF BACTERIUM

• Main Authors: TAMURA TAKASHI, MANOME ISAO, IKEDO MASASHIGE
• Format: Patent
• Language: English
• Published: 11.08.1998
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for testing bacteria in a shortened time by culturing the bacteria in the presence of an electron transfer agent and chemiluminescently detecting a reaction in which the electron transfer agent is reduced with a redoxase existing in the bacterial cells in the presence of NADH(P) as a coenzyme. SOLUTION: This method for testing bacteria comprises culturing the bacteria in the presence of an electron transfer agent comprising an oxidation type quinone such as menadione, benzoquinone, naphthoquinone, diphenoquinone or anthraquinone and detecting the reduction reaction of the electron transfer agent with a redoxase existing in the cells of the bacteria in the presence of nicotinamide adenine dinucleotide (NAPH) or nicotinamide adenine dinucleotide phosphate (NADHP) as a coenzyme by a chemiluminescent method using a luminescent reagent comprising an acridine derivative, a luminol derivative, a peroxalate ester, etc. The objective clinically important bacterium test such as an antibacterial agent sensitivity test against bacteria or a bacterium- identification test can be executed in a largely shortened time.