ESCHERICHIA COLI BETA GENE, PLANT OF FAMILY GRAMINEAE TRANSFORMED WITH THE GENE AND PRODUCTION OF THE PLANT

• Main Authors: HAYASHI YASUYUKI, TANAKA AKIRA, SHONO MARIKO, FUJIMOTO HIDEYA
• Format: Patent
• Language: English
• Published: 28.07.1998
• Edition: 6
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; TRAPPING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To obtain the subject new gene producing Oryza sativa having resistance to higher salts or drying by removing a poly(A) addition signal-like sequence and a palindrome sequence from a gene coding a natural-type Escherichia coli betA protein. SOLUTION: This new gene has a base sequence obtained by removing a poly(A) addition signal-like sequence and a palindrome sequence from a base sequence of a gene coding a natural-type Escherichia coli betA protein and codes the Escherichia coli betA protein. The gene highly manifests in a plant of family gramineae and the plant of family gramineae transformed by introducing the gene produces and accumulates glycine betaine, then the utility as a crop having resistance to higher salts stress or drying stress can be expected. The gene is obtained by collecting a gene coding a natural-type Escherichia coli betA protein and modifying its base sequence by removing a poly(A) addition signal-like sequence and a palindrome sequence by using a site-specific variation method.