MEASUREMENT OF CATALASE ACTIVITY

• Main Authors: IMAIZUMI KUNIHIRO, KUROIWA YASUYUKI, IWAMURA JUNICHI, NAKAO YOSHIKI, YAMAGATA TAKEO, YAMAKI MITSUO
• Format: Patent
• Language: English
• Published: 02.12.1997
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for measuring catalase activity excellent in reliability of a measured value. SOLUTION: In this method for measuring catalase activity by preparing a reaction solution containing a peroxide, an alcohol, a nicotinamide adenine dinucleotide, an aldehyde dehydrogenase and a specimen and determining the catalase activity from the absorbance of the reaction solution, the concentration of the peroxide in the reaction solution when the reaction solution is prepared is 4-8.3 millimol/liter, that of the alcohol is 2.4-3 mol/liter, that of the nicotinamide adenine dinucleotide is 0.71-1 millimol/liter and that of the aldehyde dehydrogenase is 800-1,000 units/liter.