MEASUREMENT OF CATALASE ACTIVITY

PROBLEM TO BE SOLVED: To provide a method for measuring catalase activity excellent in reliability of a measured value. SOLUTION: In this method for measuring catalase activity by preparing a reaction solution containing a peroxide, an alcohol, a nicotinamide adenine dinucleotide, an aldehyde dehydr...

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Bibliographic Details
Main Authors IMAIZUMI KUNIHIRO, KUROIWA YASUYUKI, IWAMURA JUNICHI, NAKAO YOSHIKI, YAMAGATA TAKEO, YAMAKI MITSUO
Format Patent
LanguageEnglish
Published 02.12.1997
Edition6
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Summary:PROBLEM TO BE SOLVED: To provide a method for measuring catalase activity excellent in reliability of a measured value. SOLUTION: In this method for measuring catalase activity by preparing a reaction solution containing a peroxide, an alcohol, a nicotinamide adenine dinucleotide, an aldehyde dehydrogenase and a specimen and determining the catalase activity from the absorbance of the reaction solution, the concentration of the peroxide in the reaction solution when the reaction solution is prepared is 4-8.3 millimol/liter, that of the alcohol is 2.4-3 mol/liter, that of the nicotinamide adenine dinucleotide is 0.71-1 millimol/liter and that of the aldehyde dehydrogenase is 800-1,000 units/liter.
Bibliography:Application Number: JP19960128771