PRODUCTION OF EXTRACELLULAR POLYSACCHARIDE PRODUCED WITH MICROORGANISM OF GENUS LIPOMYCES

• Main Authors: SHIMIZU SHIGEATSU, UEHARA AKIYOSHI, KAWAHARA YOSHIO
• Format: Patent
• Language: English
• Published: 20.05.1997
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To profitably produce the subject polysaccharide useful as a soil conditioner, a paper-strengthening agent, etc., by culturing a microorganism belonging to the genus Lipomyces and having an extracellular polysaccharide- producing ability in a culture medium, maintaining the viscosity of the culture solution at a specific value or smaller, and (semi)continuously culturing the microorganism. SOLUTION: This method for producing the extracellular polysaccharide comprises culturing and multiplying a microorganism (e.g. Lipomyces starkyi- CBS 1807) belonging to the genus Lipomyces and having an ability to produce the extracellular polysaccharide, maintaining the viscosity of the culture solution at <=150 centipoises, continuously or semi-continuously culturing the microorganism to produce and accumulate the extracellular polysaccharide in the culture solution, centrifugally removing the cells from the culture solution, concentrating the filtrate and subsequently drying the concentrated filtrate with a spray drier, etc. Thus, the objective extracellular polysaccharide comprising mannose, glucuronic acid, galactose, a trisaccharide, etc., and useful as a soil conditioner, a paper-strengthening agent, etc., is efficiently obtained.