MONOCLONAL ANTIBODY AGAINST SODIUM HYALURONATE ITS PRODUCTION

• Main Authors: SUZUKI YASUO, AKIMA KAZUO
• Format: Patent
• Language: English
• Published: 14.01.1997
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PURPOSE: To produce the subject new antibody, produced by a fused cent or a cell, capable of producing an antibody, prepared by bonding a protein, etc., to sodium hyaluronate reduced in molecular weight and immunizing a mouse with the resultant substance to a cell of a myeloma and capable of specifically recognizing the sodium hyaluronate. CONSTITUTION: A protein (e.g. hemocyanin) or a phospholipid (e.g. phosphatidylethanolamine) is introduced into sodium hyaluronate reduced in molecular weight to form a covalent combination, which is then used as an antigen and administered to immunize a BALB/C mouse therewith. Additional immunization is carried out to collect a cell of the spleen after the final immunization. The collected cell is fused to a cell of a murine myeloma in the presence of polyethylene glycol and then selectively cultured in an HAT culture medium to provide a hybridoma, which is further screened according to the enzyme- linked immunosorbent assay (ELISA), etc., to select a strain capable of producing an antibody having reactivity with sodium hyaluronate. The selected strain is cloned by a limiting dilution method and then cultured in a culture medium, etc., to afford the new monoclonal antibody against the sodium hyaluronate.