PRODUCTION OF IMMOBILIZED ENZYME

PURPOSE:To obtain a highly active immobilized enzyme for bioreactor, etc., in high efficiency by introducing an amino acid sequence having a cysteine residue at the terminal to the carboxy-terminal of an enzyme protein and immobilizing the modified protein to a carrier through an SH group. CONSTITUT...

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Bibliographic Details
Main Authors OHASHI SHINICHI, IWAKURA MASAHIRO, KOKUBU TOMOKUNI
Format Patent
LanguageEnglish
Published 06.02.1996
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE:To obtain a highly active immobilized enzyme for bioreactor, etc., in high efficiency by introducing an amino acid sequence having a cysteine residue at the terminal to the carboxy-terminal of an enzyme protein and immobilizing the modified protein to a carrier through an SH group. CONSTITUTION:This immobilized enzyme having high uniformity and activity and useful as an element of a bioreactor, etc., is produced by constructing an expression vector coding a modified enzyme protein obtained by introducing a short amino acid sequence to the carboxy terminal of an enzyme protein (e.g. dihydrofolate reductase) to form cysteine residue on the carboxy terminal of the protein, introducing the expression vector into a host cell, culturing the transformed cell to effect the expression of the gene and obtain a modified enzyme protein having an amino acid sequence composed of several number of amino acid residues having a cysteine residue terminal on the carboxy terminal of the enzyme protein and bonding the modified protein to an immobilization carrier through a mercapto group of the cysteine residue at the carboxy terminal.
Bibliography:Application Number: JP19910329540