IMMUNOASSAY OF NON-A, NON-B HEPATITIS VIRUS ASSOCIATED ANTIGEN, MONOCLONAL ANTIBODY USED THEREFOR, AND HYBRIDOMA FOR PRODUCING THIS ANTIBODY

• Main Authors: OTA YOSUKE, KASHIWAGUMA TOMIKO, YAGI SHINTARO, HASEGAWA AKIRA, KAJITA TADAHIRO, MORI HIROYUKI
• Format: Patent
• Language: English
• Published: 02.02.1996
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PURPOSE:To detect and quantitatively determine non-A, non-B hepatitis virus CORE structural protein in a specimen by producing a monoclonal antibody, which performs specific bonding to an antigen determination region, from a hybridoma cell line. CONSTITUTION:A specimen (for example, blood serum) together with polyethylene glycol is subjected to centrifuging and precipitate thereof is alkaline- processed to concentrate and decompose a virus particle. A mouse is immunized by non-A, non-B hepatitis virus CORE structural protein of this virus particle, and a lymphatic corpuscle derived from this and a myeloma cell are subjected to blending so as to obtain a hybridoma cell line. A monoclonal antibody produced by culturing this cell line is specifically bonded to an antigen determinination region on the non-A, non-B hepatitis virus CORE structural protein. Accordingly, by using this monoclonal antibody, the non-A, non-B hepatitis virus CORE structural protein can be detected and quantitatively determined.