RECOVERY OF NUCLEIC ACID OR PROTEIN FROM SAMPLE CONTAINING NUCLEAR CELL

PURPOSE: To provide a method for recovering a nuclear cell, e.g. a leukocyte from a sample such as blood without using a method such as centrifugation or filtration and taking out a nucleic acid or a protein present in the nuclear cell. CONSTITUTION: A nuclear cell is adsorbed from a sample containi...

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Bibliographic Details
Main Authors SHIBATA HIDEJI, ISHIBASHI TAKUYA, SAKURAI HIDEHIKO
Format Patent
LanguageEnglish
Published 29.10.1996
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DERIVATIVES THEREOF
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SEPARATION
SPIRITS
SUGARS
TESTING
TRANSPORTING
VINEGAR
WINE
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Summary:PURPOSE: To provide a method for recovering a nuclear cell, e.g. a leukocyte from a sample such as blood without using a method such as centrifugation or filtration and taking out a nucleic acid or a protein present in the nuclear cell. CONSTITUTION: A nuclear cell is adsorbed from a sample containing the nuclear cell by using an ultrafine fiber assembly having properties of adsorbing the nuclear cell without adsorbing an nucleate cell, e.g. an ultrafine fiber assembly having 1-5μm fiber diameter of the ultrafine fiber such as of polyester, polypropylene or nylon., and 0.1-0.4g/cm filling ratio thereof based on a container. A nucleic acid or a protein in the resultant nuclear cell is then recovered.
Bibliography:Application Number: JP19950092623