METHOD FOR PRESERVING SUSPENSION OF MICROBIAL CELL OR IMMOBILIZED MICROBIAL CELL

• Main Authors: MURAO KOZO, ENDO RYUICHI, TAMURA KOJI, HIRATA YUJI, DOI TOSHIAKI
• Format: Patent
• Language: English
• Published: 07.05.1996
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PURPOSE: To stably preserve a suspension of a microbial cell or an immobilized cell at a low cost without causing the lysis or inactivation of the enzyme at ambient temperature by suspending the microbial cell having enzymic activities or its immobilized substance in a neutral or a weak basic aqueous solution of inorganic salts at a specific concentration. CONSTITUTION: A microbial cell of a microorganism having enzymic activities such as nitrile hydratase or nitrilase activities [e.g. Gordona terrae MA-1 strain (FERM BP-4535)] is aerobically cultured in a culture medium containing an enzymic activity inducer (e.g.. 1-cyclohexenylacetonitrile) added thereto at 30 deg.C for 72hr and then centrifuged to collect the microbial cell. The resultant microbial cell or its immobilized substance is subsequently suspended in an aqueous medium comprising a neutral or a weak basic aqueous solution of inorganic salts (e.g. monopotassium phosphate) at 100mM to the saturated concentration. Thereby, the suspension of the microbial cell or immobilized microbial cell having the enzymic activities is stably preserved for a long period (e.g. 300 days) at a low cost without causing the lysis or inactivation of the enzyme even at ambient temperature.