RECOMBINANT VECTOR, TRANSFORMANT HOLDING THE SAME RECOMBINANT VECTOR AND PRODUCTION OF ANTIMICROBIAL PEPTIDE BY THE SAME TRANSFORMANT

• Main Authors: SHIMAMURA SEIICHI, KOJIMA TOMOKO, ISHIBASHI NORIO, TAKASE MITSUNORI
• Format: Patent
• Language: English
• Published: 24.10.1995
• Edition: 6
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

PURPOSE:To obtain a recombinant vector useful for readily supplying a large amount of an antimicrobial peptide (lactoferritin) having a strong antimicrobial activity. CONSTITUTION:This is a recombinant vector in which a DNA sequence coding for at least an amino acid sequence of the formula ysAX01X01X01Trp Gln X01X01 Met X02X02 (X01 is L-Ala L-Arg, etc.; X02 is L-Arg or L-Lys) is inserted and bonded to a vector, e.g. a recombinant vector p-GEXLF in which an amino acid sequence Lys Cys Phe Gln Trp Gln Arg Asn Met Arg Lys is inserted and bonded to the downstream of a colibacillus-based vector pGEX2 Tetac promoter sequence. An exemplified vector is obtained according to the scheme shown in the figure.