METHOD OF CULTURING CELL AND DEVICE THEREFOR

PURPOSE:To culture cells in high pressure by dividing a time of one batch into plural time zones corresponding to the amount of cell multiplication and taking out a culture solution in the middle of culture in these divided time zones. CONSTITUTION:A time of one batch is divided into plural time zon...

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Bibliographic Details
Main Authors NAGAI KOZO, HAYASHIDANI MASAO, HOSODA MASATAKA, HIRAMATSU MASARU, ITO HAYAMIZU, HASHIMOTO HIDEO
Format Patent
LanguageEnglish
Published 13.09.1994
Edition5
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Summary:PURPOSE:To culture cells in high pressure by dividing a time of one batch into plural time zones corresponding to the amount of cell multiplication and taking out a culture solution in the middle of culture in these divided time zones. CONSTITUTION:A time of one batch is divided into plural time zones corresponding to the amount of cell multiplication and a culture solution is taken out from the system while leaving the cells in the culture solution in the middle of culture in these divided time zones to supply a new medium to a medium dissolving tank 10. The concentration of culture products such as lactic acid to inhibit cell multiplication can be maintained low.
Bibliography:Application Number: JP19930073066