CELL ADHESION ACTIVE NEW PEPTIDE DERIVATIVE

• Main Authors: SAIKI IKUO, AZUMA ICHIRO, KUSUSE NAOTO
• Format: Patent
• Language: English
• Published: 03.06.1994
• Edition: 5
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

PURPOSE:To obtain the subject new peptide derivative, having a structure in which an aspartic acid part is cyclized and imidated and a cell adhesive activity, and useful as a cancer metastatic suppressor, a blood platelet agglutination suppressor, a wound curing agent or a bone resorption inhibitor, etc. CONSTITUTION:A cyclic peptide expressed by formula I is dissolved in methanesulfonic acid and the resultant solution is stirred at ambient temperature for 3hr. The reactional solution is then dropped into ether and stirred for 30min while being cooled with ice. The formed precipitate is further collected by filtration to provide a solid, which is then dissolved in water to remove insoluble substances by filtration. The obtained filtrate is freeze-dried to afford a crude product, which is subjected to the reversed phase high-performance liquid chromatography and eluted with a gradient of water-0.1% trifluoroacetic acid and acetonitrile-0.1% trifluoroacetic acid to afford the objective new cell adhesive active peptide derivative (salt), containing a structure expressed by formula II (A is Arg or amino acid having N in the side chain; the alpha-amino group in the amino acid residue of A may be substituted with a lower alkyl) which may be modified with a modifying group.