REAGENT FOR DETECTING SACCHARIFIED PROTEIN

PURPOSE:To obtain the title detection reagent consisting of a boronic acid derivative containing a dihydroxyboryl group bonded to an enzyme such as peroxidase or alkali phosphatase used as a labeled material and capable of readily measuring in high sensitivity by a general purpose method. CONSTITUTI...

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Bibliographic Details
Main Authors TANAKA KIMIKAZU, MIYAKE TAKEHIRO
Format Patent
LanguageEnglish
Published 09.03.1993
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE:To obtain the title detection reagent consisting of a boronic acid derivative containing a dihydroxyboryl group bonded to an enzyme such as peroxidase or alkali phosphatase used as a labeled material and capable of readily measuring in high sensitivity by a general purpose method. CONSTITUTION:One or more kinds of enzymes such as peroxidase or alkali phosphatase and a boronic acid derivative such as m-aminophenylboronic acid having a dihydroxyboryl group are dissolved in 0.5M boric acid buffer solution (pH 9.0) and both substances are bonded using glutaric aldehyde, etc., and reduced using sodium borohydride to stabilize the bond and then absorbed into an affinity chromatography using an absorbent consisting of a saccharified albumin-immobilized agarose gel and eluted with 2M acetic acid buffer solution (pH 3.0) and the eluted material is put in a cellulose tube, etc., and dialyzed to 0.25M ammonium acetate (pH 9.0) to provide the objective detection reagent for saccharified protein expressed by the formula.
Bibliography:Application Number: JP19910047733