SEPARATION OF LAVENDER-CONTAINING DYESTUFF

PURPOSE:To separate a bluish green dyestuff in high efficiency by selectively isolating a great number of protoplasts prepared by treating callus of lavender with a specific cell wall dissolving enzyme and grinding. CONSTITUTION:Callus of lavender multiplied in MS medium containing a plant hormone s...

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Bibliographic Details
Main Author OSUGE KOICHI
Format Patent
LanguageEnglish
Published 02.04.1991
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE:To separate a bluish green dyestuff in high efficiency by selectively isolating a great number of protoplasts prepared by treating callus of lavender with a specific cell wall dissolving enzyme and grinding. CONSTITUTION:Callus of lavender multiplied in MS medium containing a plant hormone such as kinetin is treated with a combined cell wall dissolving enzyme of 0.1-1.5wt.%, especially preferably 1% cellulase and 0.1-0.8%, especially preferably 0.2% pectolyase to give a great number of protoplasts, cells from which cell wall covering the outside of plant wall are removed in a viable state, which are selectively isolated. Since protoplasts are broken in a low isotonic solution such as ordinary medium or distilled water, a bluish green dyestuff contained in the cells is separated to break protoplasts. Consequently, the dyestuff is efficiently extracted.
Bibliography:Application Number: JP19890210703